![]() Sainz J, Maeda-Martínez A, Ascencio F (1998) Microb Ecol 35:188. Trabal-Fernández N, Mazón-Suástegui JM, Vázquez-Juárez R, Ascencio-valle F, Romero J (2014) Changes in the composition and diversity of the bacterial microbiota associated with oysters ( Crassostrea corteziensis, Crassostrea gigas and Crassostrea sikamea) during commercial production. Ĭáceres-Martínez J, Vásquez-Yeomans R (2013) Enfermedades, parásitos y episodios de mortalidad de ostiones de importancia comercial en México y sus implicaciones para la producción. Hidrobiológica 22(2):181–184Įlston RA, Moore J, Abbott CL (2012) Denman Island disease (causative agent Mikrocytos mackini) in a new host, Kumamoto oysters Crassostrea sikamea. Ĭáceres-Martínez J, Vásquez-Yeomans R, Guerrero-Rentería Y (2012) Early gametogenesis of Kumamoto oyster ( Crassostrea sikamea). Sekino M (2009) In search of the Kumamoto oyster Crassostrea sikamea (Amemiya, 1928) based on molecular markers: is the natural resource at stake? Fish Sci 75:819–831. Wang H, Qian L, Wang A, Guo X (2013) Occurrence and Distribution of Crassostrea sikamea (Amemiya 1928) in China. sikamea spat exposed to probiotic bacteria when compared to spat without probiotics.Ĭamara MD, Davis JP, Sekino M, Hedgecock D, Li G, Langdon CJ, Evans S (2008) The Kumamoto oyster Crassostrea sikamea is neither rare nor threatened by hybridization in the northern Ariake sea, Japan. Nonetheless, this reduction in microalgal growth observed in vitro increased growth and survival of C. calcitrans microalgae were susceptible to the presence of probiotic bacteria. sikamea spat treated with Bacillus showed significantly ( P < 0.05) higher growth and survival than the control group. ![]() The results showed a significantly ( P 0.05) compared with the control group. sikamea spat was treated for 28 days with four single/combined bacillus treatments in triplicate at a concentration of 1 × 10 6 CFU mL −1 as follows: (a) control, without treatments (b) combination of two antibiotics (10 mg L −1) (c) B. Single cultures of microalgae or bacilli served as control. subtilis (GAtB1) were individually inoculated in triplicate into 250 mL flasks containing 1 × 10 4 colony forming units (CFU) mL −1 of bacteria and 4.5 × 10 4 cell mL −1 of microalgae ( Isochrysis galbana or Chaetoceros calcitrans) to evaluate their growth during a 7-day culture. The probiotic strains Bacillus licheniformis (MAt32), B. This study assessed in vitro interaction between Bacillus bacteria and microalgae and their posterior in vivo effect on rearing Kumamoto oyster Crassostrea sikamea.
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